Inorganic phosphate regulates Glvr-1 and -2 expression: Role of calcium and ERK1/2

• Published in: Biochemical and biophysical research communications Vol. 381; no. 2; pp. 259 - 263
• Main Authors: Wittrant, Y., Bourgine, A., Khoshniat, S., Alliot-Licht, B., Masson, M., Gatius, M., Rouillon, T., Weiss, P., Beck, L., Guicheux, J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.04.2009
• Subjects: Animals; Calcium; Calcium - metabolism; Cell Line; Dental Caries - metabolism; ERK1/2; Glvr-2; Inorganic phosphate; Mice; Mitogen-Activated Protein Kinase 1 - metabolism; Mitogen-Activated Protein Kinase 3 - metabolism; Odontoblasts; Odontoblasts - drug effects; Odontoblasts - metabolism; Phosphates - pharmacology; Phosphorylation; Receptors, Virus - biosynthesis; Sodium-Phosphate Cotransporter Proteins, Type III - biosynthesis; Tooth decay; Type III sodium-dependent phosphate cotransporters Glvr-1; ERK1/2; Odontoblasts; Calcium; Inorganic phosphate; Type III sodium-dependent phosphate cotransporters Glvr-1; Glvr-2; Tooth decay
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2009.02.034

Sodium-dependent phosphate cotransporters are key regulators of phosphate homeostasis and play a major role in mineralized tissues remodelling. However, factors influencing their expression remain under consideration. In our study, modulation of type III sodium-dependent phosphate cotransporters expression by inorganic phosphate (Pi) was investigated in the murine odontoblast-like cell line MO6-G3. Experiments were designed to determine the effects of phosphate release on dental cells during tooth decay. By real-time RT-PCR we demonstrated that Glvr-1 and -2 expressions are up-regulated by Pi. The increase in Glvr-1 and -2 expressions was correlated with ERK1/2 phosphorylation and calcium/phosphate crystals formation in cultured wells. Using calcium-free culture conditions or the specific inhibitor of ERK phosphorylation (UO126), we demonstrated that Pi effects on Glvr-1 and -2 up-regulation require the presence of calcium and involve ERK signalling pathways. This study contributes to give new insights in the control of Pi transport during carious diseases.