Determination of Bile Acids in Canine Biological Samples: Diagnostic Significance

The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain fundamental for total BA measurements, there is a burg...

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Published inMetabolites Vol. 14; no. 4; p. 178
Main Authors Németh, Krisztián, Sterczer, Ágnes, Kiss, Dávid Sándor, Lányi, Réka Katalin, Hemző, Vivien, Vámos, Kriszta, Bartha, Tibor, Buzás, Anna, Lányi, Katalin
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.04.2024
Subjects
Animals
Bile
Bile acids
canine
Cholesterol
Disease prevention
Dogs
enterohepatic circulation
Food composition
Gallbladder
Health aspects
LC-MS/MS
Lipids
Liquid chromatography
Mass spectroscopy
Medical prognosis
Metabolism
Microbiota
Microorganisms
Physiological aspects
Polypeptides
Proteins
Sampling
Small intestine
Steroids
Hungary
LC-MS/MS
canine
bile acids
enterohepatic circulation
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Summary:The comprehensive examination of bile acids is of paramount importance across various fields of health sciences, influencing physiology, microbiology, internal medicine, and pharmacology. While enzymatic reaction-based photometric methods remain fundamental for total BA measurements, there is a burgeoning demand for more sophisticated techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS) for comprehensive BA profiling. This evolution reflects a need for nuanced diagnostic assessments in clinical practice. In canines, a BA assessment involves considering factors, such as food composition, transit times, and breed-specific variations. Multiple matrices, including blood, feces, urine, liver tissue, and gallbladder bile, offer insights into BA profiles, yet interpretations remain complex, particularly in fecal analysis due to sampling challenges and breed-specific differences. Despite ongoing efforts, a consensus regarding optimal matrices and diagnostic thresholds remains elusive, highlighting the need for further research. Emphasizing the scarcity of systematic animal studies and underscoring the importance of ap-propriate sampling methodologies, our review advocates for targeted investigations into BA alterations in canine pathology, promising insights into pathomechanisms, early disease detection, and therapeutic avenues.
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ISSN:2218-1989
2218-1989
DOI:10.3390/metabo14040178