Modulation of Antibody Responses against Gnathostoma spinigerum in Mice Immunized with Crude Antigen Formulated in CpG Oligonucleotide and Montanide ISA720

• Published in: Korean journal of parasitology Vol. 51; no. 6; pp. 637 - 644
• Main Authors: Intapan, P.M., Khon Kaen University, Khon Kaen 40002, Thailand, Hirunpetcharat, C., Mahidol University, Bangkok 10400, Thailand, Kularbkaew, C, Yutanawiboonchai, W, Janwan, P, Maleewong, W
• Format: Journal Article
• Language: English
• Published: Korea (South) 대한기생충학열대의학회 01.12.2013; The Korean Society for Parasitology and Tropical Medicine
• Subjects: Adjuvants, Immunologic - administration & dosage; Animals; Antibodies, Helminth - blood; Antigens, Helminth - administration & dosage; Antigens, Helminth - immunology; GNATHOSTOMA; Gnathostoma - immunology; Gnathostoma spinigerum, CpG oligonucleotide, Th1-type response, Th2-type response, nematode, immunity; Immunoglobulin G - blood; Immunoglobulin M - blood; Male; Mannitol - administration & dosage; Mannitol - analogs & derivatives; Mice; Oleic Acids - administration & dosage; Oligodeoxyribonucleotides - administration & dosage; Original; Th1 Cells - immunology; Th2 Cells - immunology; CpG oligonucleotide; Th2-type response; Gnathostoma spinigerum; Th1-type response; nematode; immunity
• ISSN: 0023-4001; 1738-0006; 1738-0006
• DOI: 10.3347/kjp.2013.51.6.637

This study aimed to investigate the antibody responses in mice immunized with Gnathostoma spinigerum crude antigen (GsAg) incorporated with the combined adjuvant, a synthetic oligonucleotide containing unmethylated CpG motif (CpG ODN 1826) and a stable water in oil emulsion (Montanide ISA720). Mice immunized with GsAg and combined adjuvant produced all antibody classes and subclasses to GsAg except IgA. IgG2a/2b/3 but not IgG1 subclasses were enhanced by immunization with CpG ODN 1826 when compared with the control groups immunized with non-CpG ODN and Montanide ISA or only with Montanide ISA, suggesting a biased induction of a Th1-type response by CpG ODN. After challenge infection with live G. spinigerum larvae, the levels of IgG2a/2b/3 antibody subclasses decreased immediately and continuously, while the IgG1 subclass remained at high levels. This also corresponded to a continuous decrease of the IgG2a/IgG1 ratio after infection. Only IgM and IgG1 antibodies, but not IgG2a/2b/3, were significantly produced in adjuvant control groups after infection. These findings suggest that G. spinigerum infection potently induces a Th2-type biased response.