Steady-state luteinizing hormone receptor messenger ribonucleic acid levels and endothelial cell composition in bovine normal- and short-lived corpora lutea

• Published in: Biology of reproduction Vol. 55; no. 4; pp. 902 - 909
• Main Authors: Smith, G.D. (The University of Chicago, Chicago, IL.), Sawyer, H.R, Mirando, M.A, Griswold, M.D, Sadhu, A, Reeves, J.J
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.10.1996
• Subjects: Animals; ARN MENSAJERO; ARN MESSAGER; Biological and medical sciences; Blotting, Northern; BOVIN DE BOUCHERIE; Cattle; Chorionic Gonadotropin - administration & dosage; CORPS JAUNE; Corpus Luteum - blood supply; Corpus Luteum - drug effects; Corpus Luteum - physiology; CUERPO LUTEO; Dinoprost - analogs & derivatives; Dinoprost - blood; Dinoprost - metabolism; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; EPITELIO; EPITHELIUM; Female; Fundamental and applied biological sciences. Psychology; GANADO DE CARNE; GONADOTROPHINE; GONADOTROPINAS; HORMONAS SINTETICAS; Hormone metabolism and regulation; HORMONE SYNTHETIQUE; Mammalian female genital system; Pregnenediones - administration & dosage; PROGESTAGENE; PROGESTAGENOS; PROGESTERONA; PROGESTERONE; Progesterone - blood; Progesterone - metabolism; Progesterone Congeners - administration & dosage; PROSTAGLANDINAS; PROSTAGLANDINE; RECEPTEUR D'HORMONE; RECEPTORES DE HORMONAS; Receptors, LH - drug effects; Receptors, LH - genetics; Receptors, LH - metabolism; RNA, Messenger - analysis; RNA, Messenger - genetics; SERUM SANGUIN; SUERO SANGUINEO; Tissue Distribution - drug effects; Tissue Distribution - physiology; VACA; VACHE; VAISSEAU SANGUIN; VASOS SANGUINEOS; Vertebrates: reproduction; Bovine; Arachidonic acid derivatives; Gonadotropin; Puerperium; Ovarian hormone; Prostaglandin F2α; Vascularization; Ovary; Vertebrata; Mammalia; Adenohypophyseal hormone; Eicosanoid; Female genital system; Corpus luteum; Female; Artiodactyla; Hormonal investigation; Progesterone; Sex steroid hormone; LH; Ungulata; Hormonal receptor
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod55.4.902

The short-lived corpus luteum (CL) contributes to reproductive inefficiency during the postpartum period in beef cows. The cause for the early demise of the short-lived CL is not fully understood but is believed to involve a premature release of prostaglandin F2alpha. The objectives of this study were to evaluate norgestomet-hCG-induced normal-lived CL and hCG-induced short-lived CL in postpartum cows with respect to serum progesterone (P4) and 13,14-dihydro-15-keto, prostaglandin F2alpha (PGFM) concentrations and luteal LH receptor (LH-R) concentrations, LH-R mRNA levels, and vascularity. Although serum P4 profiles from the time of hCG administration (Day 0) until luteectomy (Day 6, 7, or 8) were similar between CL life span groups, PGFM concentrations were elevated (p 0.05) on Day 8 in cows expected to have short-lived CL compared to normal-lived CL. The LH-R concentrations were similar between normal- and short-lived CL on all days measured. Irrespective of luteal life span and day of luteectomy, all CL possessed a 4.4-kb LH-R transcript. Actin-normalized LH-R mRNA levels were similar between normal- and short-lived CL on Days 6 and 7; however, Day 8 short-lived CL contained less (p 0.05) LH-R mRNA than Day 8 normal-lived CL. Although the area of luteal tissue occupied by capillaries in normal- and short-lived CL was similar on Days 6 and 7, the area occupied by capillaries in short-lived CL was lower (p 0.05) than that for normal-lived CL on Day 8. Collectively, these results indicate that there is a decrease in steady-state LH-R mRNA and a reduction in luteal vascularity in CL expected to be short-lived. These changes occur concomitantly with a rise in serum PGFM, but prior to a decline in serum P4