Molecular characterization of mouse 17β-hydroxysteroid dehydrogenase IV

• Published in: Journal of steroid biochemistry and molecular biology Vol. 55; no. 5; pp. 541 - 548
• Main Authors: Normand, Thierry, Husen, Bettina, Leenders, Frauke, Pelczar, Hélène, Baert, Jean-Luc, Begue, Agnès, Flourens, Anne-Claire, Adamski, Jerzy, de Launoit, Yvan
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.12.1995; Elsevier Science; Elsevier
• Subjects: 17-Hydroxysteroid Dehydrogenases - genetics; Amino Acid Sequence; Animals; Antibodies, Monoclonal - immunology; Base Sequence; Biological and medical sciences; Cloning, Molecular; Consensus Sequence; Enoyl-CoA Hydratase; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Hydro-Lyases; Isoenzymes - genetics; Life Sciences; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Molecular Weight; Multienzyme Complexes; Peroxisomal Multifunctional Protein-2; RNA, Messenger - genetics; Sequence Alignment; Sequence Homology, Amino Acid; Swine; Tissue Distribution; Vertebrata; Mammalia; Complementary DNA; Mouse; Enzyme; Rodentia; Estradiol 17β-dehydrogenase; Oxidoreductases; Molecular cloning; Gene expression
• ISSN: 0960-0760; 1879-1220
• DOI: 10.1016/0960-0760(95)00204-9

17β-hydroxysteroid dehydrogenases (17β-HSD) catalyze the conversion of estrogens and androgens at the C17 position. The 17β-HSD type I, II, III and IV share less than 25% amino acid similarity. The human and porcine 17β-HSD IV reveal a three-domain structure unknown among other dehydrogenases. The N-terminal domains resemble the short chain alcohol dehydrogenase family while the central parts are related to the C-terminal parts of enzymes involved in peroxisomal β-oxidation of fatty acids and the C-terminal domains are similar to sterol carrier protein 2. We describe the cloning of the mouse 17β-HSD IV cDNA and the expression of its mRNA. A probe derived from the human 17β-HSD IV was used to isolate a 2.5 kb mouse cDNA encoding for a protein of 735 amino acids showing 85 and 81% similarity with human and porcine 17β-HSD IV, respectively. The calculated molecular mass of the mouse enzyme amounts to 79,524 Da. The mRNA for 17β-HSD IV is a single species of about 3 kb, present in a multitude of tissues and expressed at high levels in liver and kidney, and at low levels in brain and spleen. The cloning and molecular characterization of murine, human and porcine 17β-HSD IV adds to the complexity of steroid synthesis and metabolism. The multitude of enzymes acting at C17 might be necessary for a precise control of hormone levels.