Petroleum pollution affects soil chemistry and reshapes the diversity and networks of microbial communities

• Published in: Ecotoxicology and environmental safety Vol. 246; p. 114129
• Main Authors: Li, Yongquan, Li, Caili, Xin, Ying, Huang, Tao, Liu, Jin
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.11.2022; Elsevier
• Subjects: Metabolism; Microorganism; Petroleum; Soil; Soil; Microorganism; Metabolism; Petroleum
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.114129

Soil is the bearing centre of terrestrial ecosystems. Oil pollution leads to changes in the physical and chemical properties of soil to varying degrees. Polluted soils form a unique microbial species composition, which provides rich materials for the bioremediation of oil-contaminated soil through biological enhancement. Understanding the microbial composition of petroleum-contaminated soil can provide a better biological method for soil remediation. Based on this, 16 S rRNA and ITS genetic markers were used to analyse the bacterial and fungal microbiota in petroleum-contaminated soil, and their physical and chemical properties (total organic carbon, alkaline hydrolysable nitrogen, total phosphorus, total potassium, available potassium, Cu, Zn, and Cd) were measured. It was found that petroleum pollution can significantly reduce the abundance and diversity of bacteria and fungi in the soil and significantly promote the relative abundance of Proteobacteria, Pseudomonas, Pseudoxanthomonas and Pseudoallescheria, which changed the dominant flora of bacteria and fungi and reshaped the co-occurrence network relationship between bacteria and fungi in oil-contaminated soil. The content of total organic carbon in petroleum-contaminated soil was significantly higher than that in uncontaminated soil, while the content of alkaline hydrolysable nitrogen and available potassium was significantly lower than that in uncontaminated soil, and the content of Cu significantly increased after pollution. Total organic carbon is the key driving factor that changes oil-contaminated soil microorganisms and plays a significant role in regulating the remodelling and composition of the microbial community in oil-contaminated soil. This study laid a solid theoretical foundation for the bioremediation of oil-contaminated soil. •The contents of TOC, alkaline hydrolysable nitrogen, available potassium and Cu were changed by petroleum pollution.•The alpha-diversity values of the soil bacterial and fungal communities were reduced by petroleum pollution.•Petroleum pollution affects the soil microbial community structure, biomarkers and cooccurrence network.•Proteobacteria, Pseudomonas, Pseudoxanthomonas and Pseudoallescheria had the highest relative abundances in the petroleum pollution.•TOC is the key driving factor that changes oil-contaminated soil microorganisms.