Post-thaw quality of ram sperm frozen with different concentrations of low-density lipoproteins associated with non-enzymatic antioxidants
The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with different concentrations of LDL (2 or 8%), associated with the addition...
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Published in | Animal reproduction Vol. 20; no. 1; p. e20220068 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English Portuguese |
Published |
Brazil
Colégio Brasileiro de Reprodução Animal
01.01.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with different concentrations of LDL (2 or 8%), associated with the addition of 10 mM non-enzymatic antioxidants (ascorbic acid, hydroxytoluene butylate, ascorbyl palmitate, and trehalose). Semen samples were collected from six rams, split into different treatments, and frozen. After thawing, kinematic (CASA), structural (propidium iodide and carboxyfluorescein diacetate) and functional (hypoosmotic test) sperm membrane integrity was assessed. Total motility, VCL, and LIN were also assessed in thawed samples during 3 h of incubation (38 °C). The results showed that hydroxytoluene butylate at 10 mM in Tris-Glucose extender with 8% LDL improved velocity parameters immediately post-thaw compared with Tris-Glucose egg yolk extender, as well as prevented the reduction of total motility and VCL after incubation. There was no benefit of adding ascorbic acid and trehalose. Moreover, for the first time, it was shown the motility impairment promoted by ascorbyl palmitate to ram sperm. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conflicts of interest: The authors have no conflict of interest to declare. In Memoriam Author contributions :PPNS: Conceptualization, Methodology, Supervision, Writing-review & editing; DRC: Writing- original draft, Writing-review & editing; LCOM: Data Curation, Formal Analysis, Methodology; MCS: Formal Analysis, Methodology; MMN: Conceptualization, Writing-review & editing; MRTN: Data curation, Formal Analysis; MH: Conceptualization, Funding acquisition, Supervision. |
ISSN: | 1806-9614 1984-3143 1984-3143 |
DOI: | 10.1590/1984-3143-AR2022-0068 |