Molecular cloning and expression analysis of double-stranded RNA-dependent protein kinase (PKR) in rock bream ( Oplegnathus fasciatus)

We have cloned and characterized rock bream double-stranded RNA-dependent protein kinase (PKR), which is a key component of type I IFN inducible innate immune system. Full-length rock bream PKR cDNA consists of 2115 bp ORF encoding 704 amino acids, 124 bp 5′ UTR, and 529 bp 3′ UTR. Conserved domain...

Full description

Saved in:
Bibliographic Details
Published inVeterinary immunology and immunopathology Vol. 133; no. 2; pp. 290 - 295
Main Authors Zenke, Kosuke, Nam, Yoon Kwon, Kim, Ki Hong
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.02.2010
Amsterdam: Elsevier
Subjects
Amino Acid Sequence
Animals
Base Sequence
bream
Cloning, Molecular
DNA Primers - genetics
DNA, Complementary - genetics
double-stranded RNA
eIF-2 Kinase - chemistry
eIF-2 Kinase - genetics
eIF-2 Kinase - immunology
Gene Expression
immune response
Immunity, Innate
Interferon Inducers - pharmacology
molecular cloning
Molecular Sequence Data
mRNA expression
Oplegnathus fasciatus
Perciformes - genetics
Perciformes - immunology
Perciformes - metabolism
PKR cDNA
Poly I-C - pharmacology
Poly I:C
protein kinases
Rock bream
Sequence Homology, Amino Acid
Up-Regulation - drug effects
Rock bream
PKR cDNA
Poly I:C
mRNA expression
Online AccessGet full text

Cover

More Information
Summary:We have cloned and characterized rock bream double-stranded RNA-dependent protein kinase (PKR), which is a key component of type I IFN inducible innate immune system. Full-length rock bream PKR cDNA consists of 2115 bp ORF encoding 704 amino acids, 124 bp 5′ UTR, and 529 bp 3′ UTR. Conserved domain analysis revealed that rock bream PKR contained two tandem dsRBM and kinase domain consisted of 11 kinase sub-motifs, which are characteristics found in other PKRs. Rock bream PKR was constitutively expressed in the spleen of rock bream and, upon injection of poly I:C, up-regulated not later than 12 h post injection and returned to baseline level at 48 h post injection. Although the eIF2α kinase activity of rock bream PKR was not examined in the present study, dsRNA inducible nature of rock bream PKR implies the possible important role of this gene in innate immune system of rock bream as suggested in zebrafish and flounder.
Bibliography:http://dx.doi.org/10.1016/j.vetimm.2009.08.009
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2009.08.009