Pathogenic Allodiploid Hybrids of Aspergillus Fungi

• Published in: Current biology Vol. 30; no. 13; pp. 2495 - 2507.e7
• Main Authors: Steenwyk, Jacob L., Lind, Abigail L., Ries, Laure N.A., dos Reis, Thaila F., Silva, Lilian P., Almeida, Fausto, Bastos, Rafael W., Fraga da Silva, Thais Fernanda de Campos, Bonato, Vania L.D., Pessoni, André Moreira, Rodrigues, Fernando, Raja, Huzefa A., Knowles, Sonja L., Oberlies, Nicholas H., Lagrou, Katrien, Goldman, Gustavo H., Rokas, Antonis
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 06.07.2020
• Subjects: allopolyploidy; ascomycota; aspergillosis; Aspergillus - genetics; Aspergillus - isolation & purification; cryptic species; Diploidy; Eurotiomycetes; fungal pathogen evolution; Genome, Fungal; Genomics; hybridization; Hybridization, Genetic; nonvertical evolution; pathogenicity; virulence; pathogenicity; ascomycota; nonvertical evolution; virulence; aspergillosis; Eurotiomycetes; allopolyploidy; hybridization; fungal pathogen evolution; cryptic species
• ISSN: 0960-9822; 1879-0445
• DOI: 10.1016/j.cub.2020.04.071

Interspecific hybridization substantially alters genotypes and phenotypes and can give rise to new lineages. Hybrid isolates that differ from their parental species in infection-relevant traits have been observed in several human-pathogenic yeasts and plant-pathogenic filamentous fungi but have yet to be found in human-pathogenic filamentous fungi. We discovered 6 clinical isolates from patients with aspergillosis originally identified as Aspergillus nidulans (section Nidulantes) that are actually allodiploid hybrids formed by the fusion of Aspergillus spinulosporus with an unknown close relative of Aspergillus quadrilineatus, both in section Nidulantes. Evolutionary genomic analyses revealed that these isolates belong to Aspergillus latus, an allodiploid hybrid species. Characterization of diverse infection-relevant traits further showed that A. latus hybrid isolates are genomically and phenotypically heterogeneous but also differ from A. nidulans, A. spinulosporus, and A. quadrilineatus. These results suggest that allodiploid hybridization contributes to the genomic and phenotypic diversity of filamentous fungal pathogens of humans. [Display omitted] •Aspergillus latus clinical isolates were previously misidentified as A. nidulans•A. latus is an allodiploid that arose via hybridization of two Aspergillus species•A. latus hybrid isolates exhibit heterogeneity for several infection-relevant traits•A. latus is phenotypically distinct from parental and closely related species Steenwyk, Lind, et al. identify clinical isolates of Aspergillus latus, an allodiploid species of hybrid origin. These isolates exhibit phenotypic heterogeneity in infection-relevant traits and are distinct from closely related species. The results suggest that allodiploid hybridization contributes to the evolution of filamentous fungal pathogens.