Membrane topology of the 22 kDa integral peroxisomal membrane protein

• Published in: FEBS letters Vol. 315; no. 3; pp. 217 - 222
• Main Authors: Kaldi, K., Diestelkötter, P., Stenbeck, G., Auerbach, S., Jäkle, U., Mägert, H.-J., Wieland, F.T., Just, W.W.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 11.01.1993
• Subjects: Amino Acid Sequence; Animals; Base Sequence; cDNA cloning; Cloning, Molecular; DNA; Intracellular Membranes - chemistry; liver; Liver - chemistry; membrane proteins; Membrane Proteins - chemistry; Membrane Proteins - genetics; Membrane topology; Microbodies - chemistry; Molecular Sequence Data; Peroxisomal membrane protein, 22 kDa; Peroxisome; peroxisomes; PMP, peroxisomal membrane polypeptide; PMP22 protein; PMS, post-mitochondrial supernatant; Protein Biosynthesis; Protein Conformation; Proteins - chemistry; Proteins - genetics; Rats; SDS-PAGE, sodium dodecylsulfate polyacrylamide gel electrophoresis; Sequence Homology, Amino Acid; topology; Transcription, Genetic; cDNA cloning; Peroxisomal membrane protein, 22 kDa; SDS-PAGE, sodium dodecylsulfate polyacrylamide gel electrophoresis; Peroxisome; Membrane topology; PMS, post-mitochondrial supernatant; PMP, peroxisomal membrane polypeptide
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)81167-X

In order to study the membrane topology and the possible function of the rat liver 22 kDa integral peroxisomal membrane protein (PMP 22) at a molecular level, we have cloned PMP 22 from a λgt11 expression library and sequenced its cDNA. Hydropathy analysis of the deduced primary structure indicates 4 putative transmembrane segments. The accessibility to exogenous aminopeptidase of PMP 22 in intact peroxisomes suggests that the N-terminus faces the cytosol. A model of the topology of PMP 22 in the peroxisomal membrane is discussed. Homology studies revealed a striking similarity with the Mpv 17 gene product. Lack of this membrane protein causes nephrotic syndrome in mice.