A NEW TOPOISOMERASE II INHIBITOR, BE-22179, PRODUCED BY A STREPTOMYCETE I. PRODUCING STRAIN, FERMENTATION, ISOLATION AND BIOLOGICAL ACTIVITY

• Published in: Journal of antibiotics Vol. 47; no. 2; pp. 129 - 135
• Main Authors: OKADA, HIROMASA, SUZUKI, HAJIME, YOSHINARI, TOMOKO, ARAKAWA, HIROHARU, OKURA, AKIRA, SUDA, HIROYUKI, YAMADA, AKIHIRO, UEMURA, DAISUKE
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1994; Japan Antibiotics Research Association
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Biological and medical sciences; Female; Fermentation; General pharmacology; Leukemia L1210 - drug therapy; Medical sciences; Mice; Microbial Sensitivity Tests; Peptides; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Streptomyces - chemistry; Topoisomerase II Inhibitors; Tumor Cells, Cultured - drug effects; Antineoplastic agent; DNA topoisomerase (ATP-hydrolysing); Streptomycetaceae; Enzyme; Taxonomy; Enzyme inhibitor; Streptomyces; Fermentation; In vitro; Biological activity; Actinomycetales; In vivo; Isomerases; Microbial origin; Bacteria; Isolation; Actinomycetes; Tumor cell; Physicochemical properties
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.47.129

A new topoisomerase II inhibitor, designated BE-22179, was isolated from the culture broth of Streptomyces sp. A22179, which resembles "Streptomyces gangtokensis". The inhibitor was extracted from the mycelial cake of the culture broth with organic solvent and successively purified by silica gel chromatography. BE-22179 inhibited topoisomerase II strongly but not topoisomerase I and showed potent antitumor activity against various tumor cell lines both in vitro and in vivo.