Presence of HPV type 6 in dysplasia and carcinoma arising from recurrent respiratory papillomatosis

• Published in: Head & neck Vol. 31; no. 8; pp. 1095 - 1101
• Main Authors: Jeong, Woo‐Jin, Park, Seok‐Woo, Shin, Miwha, Lee, You Jeong, Jeon, Yoon Kyung, Jung, Young Ho, Hun Hah, J., Kwon, Tack‐Kyun, Song, Yong‐Sang, Kim, Kwang Hyun, Sung, Myung‐Whun, Smith, Russell B.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.08.2009; Wiley
• Subjects: Adult; Biological and medical sciences; Biopsy, Needle; carcinoma; Cell Transformation, Neoplastic - pathology; Disease Progression; Human papillomavirus; Human papillomavirus 6 - isolation & purification; Humans; Immunohistochemistry; Infectious diseases; Laryngeal Neoplasms - pathology; Laryngeal Neoplasms - physiopathology; Laryngeal Neoplasms - virology; Male; malignant transformation; Medical sciences; Middle Aged; Neoplasm Recurrence, Local - pathology; Neoplasm Recurrence, Local - physiopathology; Neoplasm Recurrence, Local - virology; Otorhinolaryngology. Stomatology; Papilloma - pathology; Papilloma - physiopathology; Papilloma - virology; Papillomavirus Infections - diagnosis; Papillomavirus Infections - pathology; Prognosis; recurrent respiratory papillomatosis; Retrospective Studies; Risk Assessment; Sampling Studies; tumor virus infections; Viral diseases; Dysplasia; Carcinoma; Stomatology; ENT; Papovaviridae; Malignant tumor; tumor virus infections; Infection; Papillomavirus; Virus; Human papillomavirus; Recurrent respiratory papillomatosis; Malignant transformation; Viral disease; Cancer
• ISSN: 1043-3074; 1097-0347
• DOI: 10.1002/hed.20998

Background We collected rare cases of recurrent respiratory papillomatosis (RRP) undergoing malignant transformation. We sought to identify human papillomavirus (HPV) subtypes in areas of papilloma, dysplasia, and carcinoma and investigate thve pattern of protein overexpression. Methods Three patients whose disease underwent malignant transformation from RRP to carcinoma were subjected to this study. Morphologically distinct areas in the pathology specimen of each patient were diagnosed as papilloma, dysplasia, and carcinoma. Each lesion was separately obtained by laser capture microdissection and was PCR amplified for the presence of HPV. A DNA chip was used to determine the type of HPV in each area. Immunohistochemistry for p53, Ki‐67, and pRb was performed. Results HPV type 6 was present in all specimens tested positive. Expression of p53 and Ki‐67 increased with increasing severity of dysplastic change. Conclusion Although HPV type 11 is most frequently associated with malignant change of RRP, HPV type 6 may also contribute to play an equally important role in RRP carcinogenesis. © 2008 Wiley Periodicals, Inc. Head Neck, 2009