Transfection of cells with basic fibroblast growth factor and Kaposi fibroblast growth factor genes induce resistance to and receptor modulation of tumor necrosis factor

• Published in: FEBS letters Vol. 372; no. 1; pp. 44 - 48
• Main Authors: Aggarwal, Bharat B., Pocsik, Eva, Totpal, Klara
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 18.09.1995
• Subjects: 3T3 Cells; Animals; basic fibroblast growth factor; bFGF; Cell Count - drug effects; Cell Division - drug effects; Cell Size - drug effects; Cell Survival - drug effects; DMEM; Down-Regulation; Dulbecco's minimum essential medium; FBS; fetal bovine serum; Fibroblast growth factor; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - physiology; Fibroblast Growth Factor 4; Fibroblast Growth Factors - genetics; Fibroblast Growth Factors - physiology; K-fgf; kaposi sarcoma growth factor/human stomach cancer/fibroblast growth factor-5; Mice; Oncogenes; phospholipase A2; Phospholipases A - metabolism; Phospholipases A2; PLA2; Proliferation; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - physiology; Receptors, Tumor Necrosis Factor - metabolism; Resistance; TNF; Transfection - genetics; Tumor cell; Tumor necrosis factor; Tumor Necrosis Factor-alpha - pharmacology; Resistance; Fibroblast growth factor; DMEM; Tumor necrosis factor; K-fgf; FBS; Proliferation; TNF; PLA 2; Tumor cell; bFGF
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)00945-6

Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene ( bFGF) or the kaposi FGF gene ( K-fgf) on the growth-modulatory effects of TNF. Our results show that transformation of cells with either gene leads to resistance to the growth-inhibitory effects of TNF. The K-fgf gene was found to be a more potent inducer of cellular resistance than the bFGF gene. The cellular resistance correlated with the inhibition of TNF-induced activation of phospholipase A 2 and downmodulation of TNF receptors. Overall, our results indicate that both K-fgf and bFGF play an important role in suppression of antiproliferative effects of TNF.