Markedly Different Effects of Monovalent Cations on the Efficiency of Gene Expression

The effect of monovalent cations on a cell‐free transcription–translation (TX–TL) system is examined using a luciferase assay. It is found that the potency for all ions analyzed here is in the order Rb+ > K+ > Cs+ > Na+ ≈ Li+ > (CH3)4N+, where Rb+ is most efficient at promoting TX–TL and...

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Bibliographic Details
Published inAdvanced biology Vol. 7; no. 3; pp. e2200164 - n/a
Main Authors Nishio, Takashi, Masaoka, Tomoya, Yoshikawa, Yuko, Sadakane, Koichiro, Kenmotsu, Takahiro, Schiessel, Helmut, Yoshikawa, Kenichi
Format Journal Article
LanguageEnglish
Published Germany 01.03.2023
Subjects
Cations, Monovalent - pharmacology
cell‐free gene expression
Gene Expression
Lithium - pharmacology
luciferase assay
monovalent cations
Sodium - pharmacology
transcription–translation
transcription-translation
luciferase assay
monovalent cations
cell-free gene expression
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Summary:The effect of monovalent cations on a cell‐free transcription–translation (TX–TL) system is examined using a luciferase assay. It is found that the potency for all ions analyzed here is in the order Rb+ > K+ > Cs+ > Na+ ≈ Li+ > (CH3)4N+, where Rb+ is most efficient at promoting TX–TL and the ions of Li+, Na+, and (CH3)4N+ exhibit an inhibitory effect. Similar promotion/inhibition effects are observed for cell‐free TL alone with an mRNA template. Among the monovalent cations analyzed here, the effect on gene expression follows the ordering from high promotion to inhibition: Rb+ > K+ > Cs+ > Na+ ≈ Li+ > (CH3)4N+. The strongest enhancement by Rb+ suggests that it has the most suitable ionic size and hydration effect with respect to this activity.
ISSN:2701-0198
2701-0198
DOI:10.1002/adbi.202200164