Actin-binding protein is a component of bovine erythrocytes

Actin-binding protein (ABP) is a well-characterized polypeptide capable of crosslinking filamentous actin. To date, this polypeptide has been shown to exist in a number of tissues and cultured cell lines. This report shows that by using a panel of three monoclonal antibodies for immunoblotting and i...

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Bibliographic Details
Published inCell motility and the cytoskeleton Vol. 24; no. 2; p. 100
Main Authors Brown, K D, Zinkowski, R P, Hays, S E, Binder, L I
Format Journal Article
LanguageEnglish
Published United States 1993
Subjects
Animals
Antibodies, Monoclonal - immunology
Aorta
Cattle - blood
Contractile Proteins - blood
Erythrocyte Membrane - chemistry
Filamins
Immunoblotting
Isoelectric Point
Microcomputers
Microfilament Proteins - blood
Microscopy, Fluorescence
Molecular Weight
Muscle Proteins - analysis
Muscle, Smooth, Vascular - chemistry
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Summary:Actin-binding protein (ABP) is a well-characterized polypeptide capable of crosslinking filamentous actin. To date, this polypeptide has been shown to exist in a number of tissues and cultured cell lines. This report shows that by using a panel of three monoclonal antibodies for immunoblotting and immunofluorescence analysis, that ABP is present in bovine erythrocytes. Moreover, the data obtained suggest that this protein is a component of the erythrocyte membrane skeleton. Additionally, bovine erythrocyte ABP is shown to possess both an apparent molecular weight and an isoelectric point identical to that of bovine smooth muscle filamin, implying that these two polypeptides are identical.
ISSN:0886-1544
DOI:10.1002/cm.970240203