Purification of monomeric mAb from associated aggregates using selective desorption chromatography in hydroxyapatite systems

• Published in: Biotechnology and bioengineering Vol. 108; no. 4; pp. 813 - 821
• Main Authors: Morrison, Christopher J., Gagnon, Pete, Cramer, Steven M.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.04.2011; Wiley; Wiley Subscription Services, Inc
• Subjects: Adsorption; aggregates; Antibodies, Monoclonal - isolation & purification; Biological and medical sciences; Biotechnology; Ceramics; Chromatography; Chromatography, Affinity - methods; Desorption; desorption chromatography; Durapatite - chemistry; Fundamental and applied biological sciences. Psychology; Hydroxyapatite; Monoclonal antibodies; Phosphates; Proteins; selective displacement chromatography; selective displacement chromatography; Purification; Hydroxyapatite; Desorption; Selectivity; aggregates; Monoclonal antibody; Aggregate; desorption chromatography; Chromatography; monoclonal antibodies
• ISSN: 0006-3592; 1097-0290; 1097-0290
• DOI: 10.1002/bit.22971

Selective desorption on ceramic hydroxyapatite (CHT) was implemented for the purification of monomeric monoclonal antibody (mAb) from associated aggregates and other post‐protein A step impurities. A robotic liquid handling system was employed to carry out a parallel batch screen of selective desorbents on a post‐protein A step mAb mixture. The effect of different phosphate concentrations was also investigated. Selective batch separations were achieved between monomeric mAb and associated aggregates/impurities. The batch screen results also established optimal mobile phase conditions for each selective desorbent. These initial batch results were then used to guide column separations, and baseline separation of monomeric mAb from associated aggregates and impurities was achieved, validating the screening results. Selective desorption also resulted in improved separations on CHT, with 100% yield of pure monomeric mAb as compared to 61% and 79%, respectively, for conventional linear and step gradient operations. This proof of concept study demonstrates selective desorption on CHT as an effective separation technique for the purification of monomeric mAb from associated aggregates and other post‐protein A step impurities in a single process step. Biotechnol. Bioeng. 2011; 108:813–821. © 2010 Wiley Periodicals, Inc.