Isolation and structural characterization of novel sialylated oligosaccharide‐alditols from respiratory‐mucus glycoproteins of a patient suffering from bronchiectasis

• Published in: European journal of biochemistry Vol. 211; no. 3; pp. 491 - 500
• Main Authors: KLEIN, André, CARNOY, Christophe, LAMBLIN, Geneviève, ROUSSEL, Philippe, KUIK, J. Albert, VLIEGENTHART, Johannes F. G.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.1993; Blackwell
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Bronchiectasis - metabolism; Carbohydrate Sequence; Chromatography, Gel; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Fundamental and applied biological sciences. Psychology; Glycoproteins; Glycoproteins - chemistry; Humans; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Methylation; Molecular Sequence Data; Mucus - chemistry; Oligosaccharides - chemistry; Oligosaccharides - isolation & purification; Pronase - metabolism; Proteins; Sugar Alcohols - chemistry; Sugar Alcohols - isolation & purification; Human; Broncheal mucus; Mucin; Immotile cilia syndrome; FAB mass spectrum; Isolation; Primary structure; Glycoproteins; Bronchiectasis; NMR spectrum; Glycan
• ISSN: 0014-2956; 1432-1033
• DOI: 10.1111/j.1432-1033.1993.tb17575.x

The carbohydrate chains of the respiratory‐mucus glycoproteins of a patient (blood group O) suffering from bronchiectasis due to Kartagener's syndrome, were released by alkaline borohydride treatment of a pronase digest. The structures of 82 neutral and low‐molecular‐mass sialylated oligosaccharides have been described previously [van Kuik A., de Waard P., Vliegenthart J. F. G., Klein A., Carnoy C., Lamblin G. Roussel P. (1991) Eur. J. Biochem. 198, 169–182]. In the present work, medium‐size sialylated oligosaccharides were obtained after ion‐exchange chromatography and were subsequently separated into 36 fractions utilizing gel filtration, HPLC on normal‐phase alkylaminebonded silica and reverse‐phase HPLC. From these fractions, the following six sialylated hepta‐ and octa‐saccharide‐alditols have been characterized by employing 500‐MHz 1H‐NMR spectroscopy, in conjunction with fast‐atom‐bombardment mass spectroscopy and methylation analysis.