Succinate Dehydrogenase-Coenzyme Q Reductase Assay in Leucocytes

The determination of the activity of succinate dehydrogenase-coenzyme Q reductase of leucocytes mitochondria has been studied by using 2, 6-dichlorophenolindophenol as an indicator. It was found that contaminating hemoglobin, disturbed the accurate estimation of the enzyme activity in the method pre...

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Published inChemical & pharmaceutical bulletin Vol. 25; no. 10; pp. 2761 - 2762
Main Authors MASUGI, FUMINORI, SANMA, HIDEYUKI, NAKAMURA, TETSUYA
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 1977
Japan Science and Technology Agency
Subjects
electron transport
Humans
Leukocytes - enzymology
Oxidoreductases - blood
Succinate Dehydrogenase - blood
Ubiquinone
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Summary:The determination of the activity of succinate dehydrogenase-coenzyme Q reductase of leucocytes mitochondria has been studied by using 2, 6-dichlorophenolindophenol as an indicator. It was found that contaminating hemoglobin, disturbed the accurate estimation of the enzyme activity in the method previously reported. An improved assay method is presented.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.25.2761