Control of mRNA Translation by Versatile ATP-Driven Machines

• Published in: Trends in biochemical sciences (Amsterdam. Regular ed.) Vol. 44; no. 2; pp. 167 - 180
• Main Authors: Gerovac, Milan, Tampé, Robert
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2019
• Subjects: ABC proteins; Adenosine Triphosphate - metabolism; Animals; Humans; mRNA surveillance; protein translation; quality control; ribosome recycling; ribosomes; RNA, Messenger - genetics; RNA, Messenger - metabolism; translation initiation; translation termination; protein translation; ribosomes; ABC proteins; quality control; translation initiation; translation termination; mRNA surveillance; ribosome recycling
• ISSN: 0968-0004; 1362-4326
• DOI: 10.1016/j.tibs.2018.11.003

Translation is organized in a cycle that requires ribosomal subunits, mRNA, aminoacylated transfer RNAs, and myriad regulatory factors. As soon as translation reaches a stop codon or stall, a termination or surveillance process is launched via the release factors eRF1 or Pelota, respectively. The ATP-binding cassette (ABC) protein ABCE1 interacts with release factors and coordinates the recycling process in Eukarya and Archaea. After splitting, ABCE1 stays with the small ribosomal subunit and emerges as an integral part of translation initiation complexes. In addition, eEF3 and ABCF proteins control translation by binding at the E-site. In this review, we highlight advances in the fundamental role of ABC systems in mRNA translation in view of their collective inner mechanics. mRNA translation is controlled by conformational states of ABC proteins. Translation termination and initiation are linked by ABC proteins. General mechanisms apply for resetting, splitting, and recycling intermediates. ABC systems emerge as key players in antibiotic resistance and ribosome quality control.