Effects of recombinant human growth hormone on growth of human gastric carcinoma xenograft model in nude mice

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 24; pp. 3810 - 3813
• Main Author: Liang, Dao-Ming
• Format: Journal Article
• Language: English
• Published: United States Department of Emergency Surgery of the Second Affiliated Hospital, Kunming Medical College, Kunming 650101,Yunnan Province, China 28.06.2006; Baishideng Publishing Group Co., Limited
• Subjects: Animals; bcl-2-Associated X Protein - analysis; Cell Line, Tumor; Cell Proliferation - drug effects; Gastric Cancer; Human Growth Hormone - pharmacology; Human Growth Hormone - therapeutic use; Humans; Immunohistochemistry; Mice; Mice, Nude; Proliferating Cell Nuclear Antigen - analysis; Proto-Oncogene Proteins c-bcl-2 - analysis; Stomach Neoplasms - chemistry; Stomach Neoplasms - drug therapy; Stomach Neoplasms - pathology; Xenograft Model Antitumor Assays - methods; 生长因子; 胃移植; 重组体; Nude mice; Stomach neoplasm; Human growth hormone
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i24.3810

AIM： To study effects of recombinant human growth hormone （rhGH） on growth of a human gastric carcinoma cell in vivo. METHODS： Experimental mice were divided into control group, rhGH group, oxaliplatin （L-OHP） group and rhGH＋L-OHP group. Cultured human gastric carcinoma cells BGC823 were inoculated into right axilla of nude mice and carcinoma xenograft model was established successfully. Inhibitory rate of xenograft tumor growth was estimated by measuring tumor volume; expression of proliferating cell nuclear antigen （PCNA）, Bax and Bcl-2 proteins of xenograft tumor was detected using immunohistochemical S-P method. RESULTS： Tumor growth inhibitory rate, the positive expression rate of PCNA, Bax and Bcl-2 were 49.3%, 58.2%, 65.2% and 59.2% in rhGH＋L-OHP group respectively; 46.6%, 62.5%, 59.7% and 64.7% in L-OHP group; 5.0%, 82.7%, 23.2% and 82.2% in rhGH group and 0, 77.8%, 23.5% and 80.3% in control group. There was significant difference between rhGH＋L-OHP group （or L-OHP group ） and control group or rhGH group （P 〈 0.05）, whereas there were no significant differences （P 〉 0.05） between L-OHP group and rhGH＋L-OHP group and between rhGH group and control group. CONCLUSION： rhGH does not accelerate the proliferation of human gastric cancer cell in vivo.