Resveratrol and grape juice: Effects on redox status and nitric oxide production of endothelial cells in in vitro preeclampsia model

• Published in: Pregnancy hypertension Vol. 23; pp. 205 - 210
• Main Authors: Caldeira-Dias, Mayara, Viana-Mattioli, Sarah, de Souza Rangel Machado, Jackeline, Carlström, Mattias, de Carvalho Cavalli, Ricardo, Sandrim, Valéria Cristina
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2021
• Subjects: Endothelial cells; Grape juice; Medicin och hälsovetenskap; Plasma; Preeclampsia; Resveratrol; NO; Plasma; eNOS; HP; HO-1; MTT; GPX; DAF-2; DBP; Nrf2; Resveratrol; HUVEC; miRNA; GA; GSH; SBP; L-NAME; GSR; Endothelial cells; DCFH-DA; NA; ARE; PE; Preeclampsia; ROS; Grape juice
• ISSN: 2210-7789; 2210-7797; 2210-7797
• DOI: 10.1016/j.preghy.2021.01.001

•Plasma from PE patients activates ARE sequence in endothelial cells.•Resveratrol is able to potentiate ARE sequence activation in PE.•PE serum after 1h of grape juice intake induces NO production in endothelial cells.•PE serum after 1h of grape juice intake decreases antioxidant defense.•Resveratrol and grape juice have beneficial effects on endothelial cells. Preeclampsia (PE) is a hypertensive disorder of pregnancy and it is one of the main causes of maternal and fetal morbidity and mortality worldwide. It is known that oxidative stress plays a role in its pathophysiology, therefore we investigated the effects of trans-resveratrol, a potent antioxidant, on the Nrf2/ARE pathway, nitric oxide (NO) production, and reactive oxygen species (ROS) levels in an in vitro model of PE. Plasma from PE patients increased ARE activity in endothelial cells compared with plasma from healthy pregnant (HP), and the addition of resveratrol was able to potentiate this increase only in PE. Resveratrol also decreased ROS levels in the cells incubated with plasma from PE. Based on these results, we performed a pilot clinical study to compare the effects of serum from PE women before and 1 h after ingestion of polyphenol-rich whole red grapefruit juice incubated on endothelial cells, since grapefruit contains large amounts of resveratrol. Serum from PE patients, obtained one hour after juice intake, decreased antioxidants markers in cells compared with the serum before juice intake, besides, it increased NO production. In conclusion, resveratrol and polyphenol-rich red grape juice have potentially beneficial effects on endothelial cells incubated with PE plasma/serum, which could aid in the management of PE.