Evaluation of anesthesia effects on [ 18F]FDG uptake in mouse brain and heart using small animal PET

• Published in: Nuclear medicine and biology Vol. 31; no. 2; pp. 251 - 256
• Main Authors: Toyama, Hiroshi, Ichise, Masanori, Liow, Jeih-San, Vines, Douglass C., Seneca, Nicholas M., Modell, Kendra J., Seidel, Jurgen, Green, Michael V., Innis, Robert B.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2004
• Subjects: [ 18F]FDG; Anesthesia; Anesthesia - methods; Anesthetics - pharmacology; Animals; Brain; Brain - diagnostic imaging; Brain - drug effects; Brain - metabolism; Fluorodeoxyglucose F18 - pharmacokinetics; Heart; Heart - diagnostic imaging; Heart - drug effects; Isoflurane - pharmacology; Ketamine - pharmacology; Male; Mice; Mice, Inbred BALB C; Mouse; Myocardium - metabolism; Positron-Emission Tomography - methods; Radiopharmaceuticals - pharmacokinetics; Small animal PET; Xylazine - pharmacology; Heart; Small animal PET; Brain; [ 18F]FDG; Anesthesia; Mouse
• ISSN: 0969-8051; 1872-9614
• DOI: 10.1016/S0969-8051(03)00124-0

This study evaluates effects of anesthesia on 18F-FDG (FDG) uptake in mouse brain and heart to establish the basic conditions of small animal PET imaging. Prior to FDG injection, 12 mice were anesthetized with isoflurane gas; 11 mice were anesthetized with an intraperitoneal injection of a ketamine/xylazine mixture; and 11 mice were awake. In isoflurane and ketamine/xylazine conditions, FDG brain uptake (%ID/g) was significantly lower than in controls. Conversely, in the isoflurane condition, %ID/g in heart was significantly higher than in controls, whereas heart uptake in ketamine/xylazine mice was significantly lower. Results suggest that anesthesia impedes FDG uptake in mouse brain and affects FDG uptake in heart; however, the effects in the brain and heart differ depending on the type of anesthesia used.