Neuropeptide effects on rat chondrocytes and perichondrial cells in vitro

• Published in: Neuropeptides (Edinburgh) Vol. 37; no. 5; pp. 316 - 318
• Main Authors: Edoff, K., Hildebrand, C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2003; Elsevier
• Subjects: Animals; Biological and medical sciences; Calcitonin gene-related peptide; Calcitonin Gene-Related Peptide - pharmacology; Cartilage; Cartilage - cytology; Cartilage - innervation; Cartilage - metabolism; Cells, Cultured; Chondrocyte; Chondrocytes - cytology; Chondrocytes - drug effects; Chondrocytes - metabolism; Cyclic adenosine monophosphate; Cyclic AMP - metabolism; Cyclic GMP - metabolism; Cyclic guanosine monophosphate; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; MEDICIN; MEDICINE; Neurons, Afferent - metabolism; Perichondrial cell; Radioimmunoassay; Rat; Rats; Rats, Sprague-Dawley; Receptors, Calcitonin Gene-Related Peptide - antagonists & inhibitors; Sensory nerve endings; Vertebrates: endocrinology; Calcitonin gene-related peptide; Cartilage; Chondrocyte; Perichondrial cell; Sensory nerve endings; Rat; Radioimmunoassay; Cyclic guanosine monophosphate; Cyclic adenosine monophosphate; Vertebrata; Mammalia; Animal; Rodentia; In vitro; Neuropeptide
• ISSN: 0143-4179; 1532-2785; 1532-2785
• DOI: 10.1016/j.npep.2003.09.001

This study examines if cultured chondrocytes and perichondrial cells change the level of cAMP and/or cGMP in response to application of the neuropeptide calcitonin gene-related peptide (CGRP). Cells collected from the knee region of 4–8 days old rat pups were cultured in vitro. Cultures were exposed to 10 −10–10 −6 M CGRP during 10 minutes. The levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in the cultures and in controls were determined by radioimmunoassay. The results show that application of CGRP causes a distinctly increased level of cAMP, that was absent when CGRP was applied together with the CGRP 1 receptor antagonist. The level of cGMP was not obviously altered. Hence, it is possible that terminals of primary sensory neurones present in developing cartilage influence chondrocytes and perichondrial cells via local release of CGRP.