Human serum albumin hydropersulfide is a potent reactive oxygen species scavenger in oxidative stress conditions such as chronic kidney disease

• Published in: Biochemical and biophysical research communications Vol. 479; no. 3; pp. 578 - 583
• Main Authors: Shibata, Akitomo, Ishima, Yu, Ikeda, Mayumi, Sato, Hirokazu, Imafuku, Tadashi, Chuang, Victor.T.G., Ouchi, Yuya, Abe, Takaya, Watanabe, Hiroshi, Ishida, Tatsuhiro, Otagiri, Masaki, Maruyama, Toru
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.10.2016
• Subjects: 60 APPLIED LIFE SCIENCES; Adult; Aged; Aged, 80 and over; ALBUMINS; Animals; antioxidant activity; BLOOD SERUM; Chronic kidney disease; cysteine; DISEASES; Female; fluorescent dyes; Fluorescent Dyes - chemistry; Free Radical Scavengers - chemistry; Hemodialysis; Human serum albumin; Humans; Hydropersulfides; kidney diseases; KIDNEYS; Male; Middle Aged; MOLECULAR WEIGHT; NATIONAL DEFENSE; Oxidants - chemistry; OXIDATION; Oxidative Stress; RATS; Rats, Wistar; Reactive oxygen species; Reactive Oxygen Species - metabolism; Renal Dialysis; Renal Insufficiency, Chronic - metabolism; Renal Insufficiency, Chronic - therapy; Serum Albumin - chemistry; Spectrometry, Mass, Electrospray Ionization; spectroscopy; STRESSES; Sulfhydryl Compounds - chemistry; Sulfides - chemistry; sulfur; thiols; tissues; RSSH; Human serum albumin; Reactive oxygen species; Hemodialysis; HSA; H2S; Chronic kidney disease; Hydropersulfides; HD; CKD
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2016.09.113

Recently, hydropersulfide (RSSH) was found to exist in mammalian tissues and fluids. Cysteine hydropersulfide can be found in free cysteine residues as well as in proteins, and it has potent antioxidative activity. Human serum albumin (HSA) is the most abundant protein in mammalian serum. HSA possesses a free thiol group in Cys-34 that could be a site for hydropersulfide formation. HSA hydropersulfide of high purity as a positive control was prepared by treatment of HSA with Na2S. The presence of HSA hydropersulfide was confirmed by spectroscopy and ESI-TOFMS analysis where molecular weights of HSA hydropersulfide by increments of approximately 32 Da (Sulfur atom) were detected. The fluorescent probe results showed that Alexa Fluor 680 conjugated maleimide (Red-Mal) was a suitable assay and bromotrimethylammoniumbimane bromide appeared to be a selective reagent for hydropersulfide. The effect of oxidative stress related disease on the existence of albumin hydropersulfides was examined in rat 5/6 nephrectomy model of chronic kidney disease (CKD). Interestingly, the level of hydropersulfides in rat 5/6 nephrectomy model serum was decreased by a uremic toxin that increases oxidative stress in rat 5/6 nephrectomy model. Furthermore, we demonstrated that the levels of HSA hydropersulfide in human subjects were reduced in CKD but restored by hemodialysis using Red-Mal assay. We conclude that HSA hydropersulfide could potentially play an important role in biological anti-oxidative defense, and it is a promising diagnostic and therapeutic marker of oxidative diseases. •Hydropersulfide can behave as potent antioxidants.•We firstly detected human serum albumin hydropersulfide in healthy subjects.•Human serum albumin hydropersulfide in human subjects were reduced in chronic kidney disease but restored by hemodialysis.