“Decoding the Dots”: The ImageStream system (ISS) as a novel and powerful tool for flow cytometric analysis

The aim of this article is to provide a brief review of the ImageStream system (ISS). The ISS technology was developed as a novel method for multiparameter cell analysis and subsequently as a supportive tool for flow cytometry (FC). ISS integrates the features of FC and fluorescent microscopy collec...

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Bibliographic Details
Published inCentral European journal of biology Vol. 3; no. 1; pp. 1 - 10
Main Authors Zuba-Surma, Ewa K., Kucia, Magdalena, Ratajczak, Mariusz Z.
Format Journal Article
LanguageEnglish
Published Warsaw Versita 01.03.2008
De Gruyter
Subjects
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Flow cytometry
Human Genetics
ImageStream
Immunology
Life Sciences
Peripheral blood
Review Article
Flow cytometry
ImageStream
Peripheral blood
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Summary:The aim of this article is to provide a brief review of the ImageStream system (ISS). The ISS technology was developed as a novel method for multiparameter cell analysis and subsequently as a supportive tool for flow cytometry (FC). ISS integrates the features of FC and fluorescent microscopy collecting images of acquired cells for offline digital image analysis. The article presents an overview of the main characteristics of ISS and a comparison between ISS, FC and the laser scanning cytometer (LSC). We reviewed ISS applications focusing on those involved in cellular phenotyping and provide our own experience with using ISS as a supportive tool to classical FC and demonstrate the compatibility between FC and ISS photometric analysis as well as the advantages of using ISS to confirm FC results.
ISSN:1895-104X
2391-5412
1644-3632
2391-5412
DOI:10.2478/s11535-007-0044-8