Reconstituted basement membrane enables airway epithelium modeling and nanoparticle toxicity testing
Basement membrane (BM) acts as a sheet-like extracellular matrix to support and promote the formation of epithelial and endothelial cell layers. The in vitro reconstruction of the BM is however not easy due to its ultrathin membrane features. This difficulty is overcome by self-assembling type IV co...
Saved in:
Published in | International journal of biological macromolecules Vol. 204; pp. 300 - 309 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
15.04.2022
Elsevier |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Basement membrane (BM) acts as a sheet-like extracellular matrix to support and promote the formation of epithelial and endothelial cell layers. The in vitro reconstruction of the BM is however not easy due to its ultrathin membrane features. This difficulty is overcome by self-assembling type IV collagen and laminin in the porous areas of a monolayer of crosslinked gelatin nanofibers deposited on a honeycomb microframe. Herein, a method is presented to generate airway epithelium by using such an artificial basement membrane (ABM) and human-induced pluripotent stem cells (hiPSCs). Bipolar primordial lung progenitors are firstly induced from hiPSCs and then replated on the ABM for differentiation toward matured airway epithelium under submerged and air-liquid interface culture conditions. As a result, a pseudostratified airway epithelium consisting of several cell types is achieved, showing remarkable apical secretion of MUC5AC proteins and clear advantages over other types of substrates. As a proof of concept, the derived epithelium is used for toxicity test of cadmium telluride (CdTe) nanoparticles (NPs), demonstrating the applicability of ABM-based assays involving hiPSC-derived epithelial cells-based assays.
[Display omitted]
•Generation of airway epithelium by using reconstituted basement membrane and human induced pluripotent stem cells.•Maturation of the airway epithelial cells under submerged and air-liquid interface culture conditions.•Observation of pseudostratified organization involving goblet, ciliated, and basal cells.•Demonstration of CdTe nanoparticle toxicity with the fabricated airway epithelium. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-8130 1879-0003 |
DOI: | 10.1016/j.ijbiomac.2022.02.018 |