Patterns of soybean proline-rich protein gene expression

• Published in: The Plant cell Vol. 4; no. 1; pp. 99 - 110
• Main Authors: Wyatt, R.E. (University of Georgia, Athens, GA), Nagao, R.T, Key, J.L
• Format: Journal Article
• Language: English
• Published: England American Society of Plant Physiologists 01.01.1992
• Subjects: ARN MENSAJERO; ARN MESSAGER; Cell walls; COTILEDONES; COTYLEDON; Epidermal cells; EPIDERME; EPIDERMIS; EXPRESION GENICA; EXPRESSION DES GENES; FEUILLE; FLOEMA; GENE; GENES; GLYCINE MAX; Glycine max - genetics; GRAINE; HIPOCOTILOS; Histocytochemistry - methods; HOJAS; HYPOCOTYLE; Hypocotyls; In situ hybridization; Messenger RNA; Multigene Family; Nucleic Acid Hybridization; PARED CELULAR; PAROI CELLULAIRE; Peptide Biosynthesis; Peptides; Phloem; PHLOEME; Plant cells; Plant Proteins - biosynthesis; PROLINA; PROLINE; Proline-Rich Protein Domains; PROTEINAS VEGETALES; PROTEINE VEGETALE; RNA, Messenger - isolation & purification; Seedlings; SEMILLA; Soybeans; TESTA; Tissue Distribution; XILEMA; XYLEME
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.4.1.99

The expression patterns of three members of a gene family that encodes proline-rich proteins in soybean (SbPRPs) were examined using in situ hybridization experiments. In most instances, the expression of SbPRP genes was intense in a limited number of cell types of a particular organ. SbPRP1 RNA was localized in several cell types of soybean hypocotyls, including cells within the phloem and xylem. SbPRP1 expression increased within epidermal cells in the elongating and mature regions of the hypocotyl; expression was detected also in lignified cells surrounding the hilum of mature seeds. SbPRP2 RNA was present in cortical cells and in the vascular tissue of the hypocotyl, especially cells of the phloem. This gene was expressed also in the inner integuments of the mature seed coat. SbPRP3 RNA was localized specifically to the endodermoid layer of cells surrounding the stele in the elongating region of the hypocotyl, as well as in the epidermal cells of leaves and cotyledons. These data show that members of this gene family exhibit cell-specific expression. The members of the SbPRP gene family are expressed in different types of cells and in some cell types that also express the glycine-rich protein or hydroxyproline-rich glycoprotein classes of genes