New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

• Published in: Virus research Vol. 241; pp. 88 - 94
• Main Authors: Desbiez, C., Wipf-Scheibel, C., Millot, P., Verdin, E., Dafalla, G., Lecoq, H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.09.2017; Elsevier
• Subjects: Cucurbit; Evolution; Interspecific recombination; Life Sciences; Microbiology and Parasitology; Phytopathology and phytopharmacy; Potyvirus; Vegetal Biology; Virology; Evolution; Cucurbit; Potyvirus; Interspecific recombination; species diversification; new species; cucurbitacees; pathologie végétale; phytopathogenic virus; soudan; potyvirus; evolution; recombinaison génétique; culture légumière de plein champ; papaya ringspot virus; diversification des espèces; interspecific recombination; virus de la tache annulaire de la papaye; phylogeny; phylogénie; cucurbit; espèce nouvelle; virus phytopathogène
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2017.06.022

•Two new potyvirus species from Sudan were defined in the PRSV cluster.•SuWMV is an interspecific recombinant in the 5′ part of its genome.•The PRSV cluster may have radiated about 3600 years ago.•The PRSV cluster underwent an important diversification in Africa. The “Papaya ringspot virus (PRSV) cluster” of cucurbit-infecting potyviruses contains five acknowledged species that have similar biological, serological and molecular properties. Additional data suggest there are other uncharacterized species from various locations in the world that likely belong to the PRSV cluster including a new PRSV-like virus reported from Sudan in 2003. Molecular and biological data indicated that the virus from Sudan belongs to a new species, tentatively named wild melon vein banding virus (WMVBV). The complete nucleotide sequence of a second virus from Sudan revealed it was a divergent relative of Moroccan watermelon mosaic virus (MWMV). Based on sequence similarity this virus was determined to be a distinct species and tentatively named Sudan watermelon mosaic virus (SuWMV). Molecular analyses indicate that SuWMV is a recombinant between WMVBV- and MWMV-related viruses. Based on surveys performed in Sudan between 1992 and 2012, SuWMV appeared 10 times more frequent than WMVBV in that country (14.6% vs. 1.5% of the samples tested). The geographic structure and molecular diversity patterns of the putative and acknowledged species suggest that the PRSV-like cluster originated in the Old World about 3600 years ago, with an important diversification in Africa.