Effect of glutamine on liver injuries induced by intestinal ischemia-reperfusion in rats

• Published in: Nutrición hospitalaria : organo oficial de la Sociedad Española de Nutrición Parenteral y Enteral Vol. 34; no. 3; pp. 548 - 554
• Main Authors: Hartmann, Renata, Licks, Francielli, Schemitt, Elizângela Gonçalves, Colares, Josieli Raskopf, Da Silva, Juliana, Moura, Rosa Maria, Zabot, Gilmara Pandolfo, Fillmann, Henrique Sarubbi, Marroni, Norma Possa
• Format: Journal Article
• Language: English
• Published: Spain Grupo Arán 05.06.2017
• Subjects: Animals; Glutamine - therapeutic use; Inflammation - blood; Inflammation - prevention & control; Intestinal Diseases - drug therapy; Intestinal Diseases - etiology; Liver - injuries; Liver Function Tests; Male; Nutrition & Dietetics; Rats; Rats, Wistar; Reperfusion Injury - complications; Reperfusion Injury - drug therapy; DNA damage. Inflammation. Lipid peroxidation. Oxidative stress; Oxidative stress; Inflamación; DNA damage; Daño en el DNA; Peroxidación lipídica; Lipid peroxidation; Inflammation; Estrés oxidativo
• ISSN: 0212-1611; 1699-5198; 1699-5198
• DOI: 10.20960/nh.643

Intestinal ischemia-reperfusion (I/R) injury may cause cell and tissue damage, reaching also other organs such as the liver. Because of the involvement of free radicals in I/R injury, treatment options with antioxidants have been studied and tested. To evaluate the effect of glutamine (Gln) in the liver of animals with intestinal I/R injury. We used 20 male Wistar rats divided into four groups: sham-operated (SO); glutamine + sham-operated (G+SO); intestinal ischemia-reperfusion (I/R); glutamine + intestinal ischemia-reperfusion (G+I/R). The superior mesenteric artery was clamped for 30 minutes and reperfused for 15 minutes. Gln (25 mg/kg/day) diluted in 1 ml of saline was administered intraperitoneally on the two days before I/R induction. Levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), lipid peroxidation (LPO) and expressions of interleukin-6 (IL-6) and nuclear factor kappa B (NF-kB) showed a significant reduction in the G+I/R group as compared with the I/R group. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and the levels of glutathione (GSH) showed an increase in the G+I/R group as compared with the I/R group. Pretreatment with Gln reduced oxidative, tissue damage and showed a decrease expression of inflammatory mediators.