Improving resolution of second harmonic generation microscopy via scanning structured illumination

Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SH...

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Published inBiomedical optics express Vol. 9; no. 12; pp. 6081 - 6090
Main Authors Yeh, Chia-Hua, Tan, Cheng-Zn, Cheng, Ching-hsiao Arthur, Hung, Jui-Ting, Chen, Szu-Yu
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.12.2018
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Summary:Second harmonic generation microscopy (SHGM) is a well-known technique for examining the noncentrosymmetric structures in biomedical research. However, without real-state transitions, fluorescence-based superresolution methods cannot be applied. To improve the resolution, fringe-scanning SHGM (FS-SHGM), which combines SHGM with structured illumination based on point-scanning, is introduced in this paper. The scanning path was modulated to generate illumination patterns. For the coherent parts of SHG signals, a mathematical model of image formation and reconstruction was established. Both simulations and experiments showed a resolution improvement factor of ~1.4 in the lateral and 1.56 in the axial directions for chicken tendons and mouse skin.
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ISSN:2156-7085
2156-7085
DOI:10.1364/BOE.9.006081