A preliminary X-ray study of murine Tnfaip8/Oxi-α

• Published in: International journal of molecular sciences Vol. 15; no. 3; pp. 4523 - 4530
• Main Authors: Lee, Daeun, Kim, Mi-Sun, Park, Jimin, Jhon, Gil-Ja, Son, Jin H, Shin, Dong Hae
• Format: Journal Article
• Language: English
• Published: Switzerland Molecular Diversity Preservation International (MDPI) 14.03.2014; MDPI AG
• Subjects: Animals; Apoptosis Regulatory Proteins - chemistry; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; C165S mutant; Crystallization; Crystallography, X-Ray; Electrophoresis, Polyacrylamide Gel; Mice; Mutant Proteins - chemistry; Mutant Proteins - genetics; Mutant Proteins - metabolism; Mutation, Missense; Oxi-α; oxidative stress; Parkinson’s disease; Synchrotrons; X-ray crystallography
• ISSN: 1422-0067; 1422-0067
• DOI: 10.3390/ijms15034523

Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 Å using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P21212, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.