Meyerozyma caribbica Isolated from Vinasse-Irrigated Sugarcane Plantation Soil: A Promising Yeast for Ethanol and Xylitol Production in Biorefineries

• Published in: Journal of fungi (Basel) Vol. 9; no. 8; p. 789
• Main Authors: Alencar, Bárbara Ribeiro Alves, de Freitas, Renan Anderson Alves, Guimarães, Victor Emanuel Petrício, Silva, Rayssa Karla, Elsztein, Carolina, da Silva, Suzyanne Porfírio, Dutra, Emmanuel Damilano, de Morais Junior, Marcos Antonio, de Souza, Rafael Barros
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26.07.2023; MDPI
• Subjects: Acetic acid; Alternative energy sources; Bagasse; Biodiesel fuels; Biofuels; Biomass; Cactus; Cellulose; Chemical oxygen demand; Distilleries; Drug tolerance; Ethanol; Fermentation; Glucose; hydrolysate; Hydrolysates; Lignocellulose; Meyerozyma caribbica; Microorganisms; mixed substrate; Molasses; non-conventional yeast; Potassium; Sorghum; Sugarcane; Syrups & sweeteners; Xylitol; Xylose; Yeast; Brazil
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof9080789

The production of fuels and other industrial products from renewable sources has intensified the search for new substrates or for the expansion of the use of substrates already in use, as well as the search for microorganisms with different metabolic capacities. In the present work, we isolated and tested a yeast from the soil of sugarcane irrigated with vinasse, that is, with high mineral content and acidic pH. The strain of Meyerozyma caribbica URM 8365 was able to ferment glucose, but the use of xylose occurred when some oxygenation was provided. However, some fermentation of xylose to ethanol in oxygen limitation also occurs if glucose was present. This strain was able to produce ethanol from molasses substrate with 76% efficiency, showing its tolerance to possible inhibitors. High ethanol production efficiencies were also observed in acidic hydrolysates of each bagasse, sorghum, and cactus pear biomass. Mixtures of these substrates were tested and the best composition was found for the use of excess plant biomass in supplementation of primary substrates. It was also possible to verify the production of xylitol from xylose when the acetic acid concentration is reduced. Finally, the proposed metabolic model allowed calculating how much of the xylose carbon can be directed to the production of ethanol and/or xylitol in the presence of glucose. With this, it is possible to design an industrial plant that combines the production of ethanol and/or xylitol using combinations of primary substrates with hydrolysates of their biomass.