A novel bioremediation strategy for petroleum hydrocarbon pollutants using salt tolerant Corynebacterium variabile HRJ4 and biochar

• Published in: Journal of environmental sciences (China) Vol. 47; no. 9; pp. 7 - 13
• Main Authors: Zhang, Hairong, Tang, Jingchun, Wang, Lin, Liu, Juncheng, Gurav, Ranjit Gajanan, Sun, Kejing
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2016
• Subjects: Biochar; Biodegradation, Environmental; Bioremediation; Charcoal - chemistry; China; Corynebacterium - physiology; Corynebacterium variabile HRJ4; Immobilization; Petroleum - analysis; Petroleum - metabolism; Petroleum hydrocarbons; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - metabolism; Salt-Tolerance; Soil Pollutants - analysis; Soil Pollutants - metabolism; 修复策略; 基因序列分析; 棒状杆菌; 生物炭; 石油烃类污染物; 石油烃降解; 细菌分离; 耐盐性; Petroleum hydrocarbons; Immobilization; Corynebacterium variabile HRJ4; Bioremediation; Biochar
• ISSN: 1001-0742; 1878-7320
• DOI: 10.1016/j.jes.2015.12.023

The present work aimed to develop a novel strategy to bioremediate the petroleum hydrocarbon contaminants in the environment.Salt tolerant bacterium was isolated from Dagang oilfield,China and identified as Corynebacterium variabile HRJ4 based on 16 S r RNA gene sequence analysis.The bacterium had a high salt tolerant capability and biochar was developed as carrier for the bacterium.The bacteria with biochar were most effective in degradation of n-alkanes（C16,C18,C19,C26,C28） and polycyclic aromatic hydrocarbons（NAP,PYR） mixture.The result demonstrated that immobilization of C.variabile HRJ4 with biochar showed higher degradation of total petroleum hydrocarbons（THPs） up to 78.9%after 7-day of incubation as compared to the free leaving bacteria.The approach of this study will be helpful in clean-up of petroleum-contamination in the environments through bioremediation process using eco-friendly and cost effective materials like biochar.