Evaluation of the performance of commercially available polysaccharide-based chiral stationary phases after multicycle operation in multimodal elution mode

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 39; no. 3; pp. 425 - 430
• Main Authors: Török, Gabriella, Goetelen, Ludwig, Luyckx, Raf, Broeck, Peter Van
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.09.2005; Elsevier Science
• Subjects: Acetonitriles - chemistry; Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemistry, Pharmaceutical - methods; Chiral HPLC; Chromatography; Chromatography, High Pressure Liquid; Commercially available polysaccharide-based chiral stationary phases; Drug Industry - methods; Electrodes; Ethanol - chemistry; Fundamental and applied biological sciences. Psychology; General pharmacology; Heptanes - chemistry; Hydrogen-Ion Concentration; Kinetics; Medical sciences; Methanol - chemistry; Models, Chemical; Multimodal elution mode; Performance of the columns; Pharmacology. Drug treatments; Polysaccharides - chemistry; Stereoisomerism; Temperature; Time Factors; Chiral HPLC; Commercially available polysaccharide-based chiral stationary phases; Multimodal elution mode; Performance of the columns; Evaluation; HPLC chromatography; Polysaccharide
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2005.02.020

The performance of four commercially available polysaccharide-based chiral stationary phases, Chiralcel OD, Chiralcel OJ, Chiralpak AD and Chiralpak AS was evaluated after several cycles of extended multimodal operations. Acetonitrile, methanol, ethanol and their mixtures were chosen for polar-organic mobile phases, ethanol– n-heptane mixture and n-heptane were selected for normal-phase mode. Retention factor ( k), selectivity ( α), resolution ( R s) and theoretical plate count ( N) were the chosen parameters to describe the column performance. One racemate for which all four columns have shown enantioselectivity was chosen as test compound. After 15 cycles of multimodal operations a slight decrease was seen in the retention factors ( k) however, column efficiency, selectivity ( α) and resolution ( R s) were maintained.