Cobalt tetrasulphonated phthalocyanine immobilized on poly- l-lysine film onto glassy carbon electrode as amperometric sensor for cysteine

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 42; no. 2; pp. 184 - 191
• Main Authors: Luz, Rita de Cássia Silva, Moreira, Adriano Benedito, Damos, Flavio Santos, Tanaka, Auro Atsushi, Kubota, Lauro Tatsuo
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 18.09.2006; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Carbon; Cobalt tetrasulphonated phthalocyanine; Cysteine; Cysteine - analysis; Electrochemistry - instrumentation; Electrochemistry - methods; Electrodes; Fundamental and applied biological sciences. Psychology; General pharmacology; Indoles - chemistry; Medical sciences; Organometallic Compounds - chemistry; Pharmacology. Drug treatments; Poly- l-lysine; Polylysine - chemistry; Reproducibility of Results; Sensitivity and Specificity; Cysteine; Cobalt tetrasulphonated phthalocyanine; Poly- l-lysine; Cobalt Complexes; Thiol; Immobilization; Lysine polymer; Polyaminoacid; Transition metal Complexes; Carbon; Sulfur containing aminoacid; Electrodes; Poly-L-lysine; Metallophthalocyanine; Amperometry; Glassy state
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2006.03.036

The present work describes the development of a simple and efficient method for the amperometric determination of cysteine (CySH) in wild medium at an applied potential of 0.150 V versus Ag/AgCl. In this sense, the electrocatalytic oxidation of cysteine (CySH) was carried out on a glassy carbon electrode modified with cobalt tetrasulphonated phthalocyanine (CoTSPc) and poly- l-lysine (PLL) film. The immobilization of CoTSPc in PLL film was performed by a simple evaporation of the solvent. The CoTSPc/PLL film, formed on the GC electrode showed an electrocatalytic activity to the CySH oxidation. The sensor presented its best performance in 0.1 mol l −1 Pipes at pH 7.5. Under optimized operational conditions, the sensor provided a wide linear response range for CySH from 0.50 up to 216.0 μmol l −1 with a sensitivity and detection limit of 157 nA cm −2 l μmol −1 and 150 nmol l −1, respectively. The proposed sensor presented higher sensitivity when compared to the other modified electrodes described in the literature and showed a stable response for at least 200 successive determinations. The repeatability of the measurements with the same sensor and different sensors, evaluated in term of relative standard deviation, were 4.1 and 5.2%, respectively, for n = 10. The developed sensor was applied for the CySH determination in food supplement samples and the results were statistically the same to those obtained by a comparative method described in the literature at a confidence level of 95%.