Phosphorylated vasodilator-stimulated phosphoprotein is localized on mitotic spindles of the gastric cancer cell line SGC-7901

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 46; pp. 7478 - 7481
• Main Authors: Tao, Yan, Chen, Yong-Chang, Wang, Ying, Zhang, Zhi-Jian, Xu, Wen-Rong
• Format: Journal Article
• Language: English
• Published: United States School of Medicine, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China%School of Medical Technology, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China 14.12.2006; Baishideng Publishing Group Co., Limited
• Subjects: Cell Adhesion Molecules - chemistry; Cell Adhesion Molecules - metabolism; Cell Line, Tumor; Gastric Cancer; Humans; Microfilament Proteins - chemistry; Microfilament Proteins - metabolism; Microscopy, Fluorescence; Phosphoproteins - chemistry; Phosphoproteins - metabolism; Phosphorylation; Spindle Apparatus - metabolism; Stomach Neoplasms - metabolism; Tubulin - metabolism; 有丝分裂; 磷酸化; 纺锤体; 胃癌细胞系; 血管扩张剂刺激磷蛋白; Localization; Mitotic spindle; Phosphorylated vasodilator-stimulated phosphoprotein; Gastric cancer cell
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i46.7478

AIM： To elucidate the localization of vasodilator stimulated phosphoprotein （VASP）, a cytoskeletal organizing protein and a substrate of protein kinases A and G in mitotic gastric cancer cells. METHODS： Immunofluorescence microscopy was used to observe the localization of α-tubulin, VASP and Ser157 phosphorylated VASP （p-VASP） in interphase of mitotic gastric cancer of the cell line SGC-7901. RESULTS： Immunofluorescence staining showed that p-VASP but not VASP was co-localized with α-tubulin on spindle poles and fibers in prophase, metaphase and anaphase of the mitotic process of the gastric cancer cell line SGC-7901. H89, an inhibitor of protein kinases A and G, had no effect on the localization of p-VASP on the spindles. CONCLUSION： VASP may play a role in assembling and stabilizing the mitotic spindle of cells, and phosphorylation of the protein is the precondition for it to exert this function.