Canonical Wnts, specifically Wnt-10b, show ability to maintain dermal papilla cells

• Published in: Biochemical and biophysical research communications Vol. 438; no. 3; pp. 493 - 499
• Main Authors: Ouji, Yukiteru, Nakamura-Uchiyama, Fukumi, Yoshikawa, Masahide
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.08.2013
• Subjects: 60 APPLIED LIFE SCIENCES; Animals; Canonical pathway; CELL PROLIFERATION; Cell Proliferation - drug effects; Cells, Cultured; Cercopithecus aethiops; COS Cells; Dermal papilla; Dermis - cytology; Hair Follicle - cytology; Hair Follicle - metabolism; HAIR FOLLICLES; HYPERFINE STRUCTURE; IN VITRO; IN VIVO; Mice; Mice, Transgenic; TRANSGENIC MICE; Wnt Proteins - physiology; Wnt signaling; Wnt-10b; Wnt3A Protein - physiology; Wnt-10b; Wnt signaling; Dermal papilla; Canonical pathway
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2013.07.108

•First report on effects of various Wnts on DP cells.•Wnt-10b promoted trichogenesis, while Wnt-3a showed to a limited extent.•Canonical Wnts, specifically Wnt-10b, is important for DP cells maintenance. Although Wnts are expressed in hair follicles (HFs) and considered to be crucial for maintaining dermal papilla (DP) cells, the functional differences among them remain largely unknown. In the present study, we investigated the effects of Wnts (Wnt-3a, 5a, 10b, 11) on the proliferation of mouse-derived primary DP cells in vitro as well as their trichogenesis-promoting ability using an in vivo skin reconstitution protocol. Wnt-10b promoted cell proliferation and trichogenesis, while Wnt-3a showed those abilities to a limited extent, and Wnt-5a and 11 had no effects. Furthermore, we investigated the effects of these Wnts on cultured DP cells obtained from versican-GFP transgenic mice and found that Wnt-10b had a potent ability to sustain their GFP-positivity. These results suggest that canonical Wnts, specifically Wnt-10b, play important roles in the maintenance of DP cells and trichogenesis.