Comparative analysis of reverse-transcription-polymerase chain reaction for Aichivirus detection

• Published in: Food science and biotechnology Vol. 33; no. 12; pp. 2807 - 2814
• Main Authors: Kim, Mi-Gyeong, Yoo, Boeun, Min, A Young, Seo, Doo Won, Choi, Changsun, Kim, Seung Hwan, Kim, Soon Han
• Format: Journal Article
• Language: English
• Published: Singapore Springer Nature Singapore 01.09.2024; Springer Nature B.V; 한국식품과학회
• Subjects: Aquatic environment; Chemistry; Chemistry and Materials Science; Comparative analysis; detection limit; Detection limits; family; Feces; Food chains; Food contamination; Food Science; Gastroenteritis; genus; Japan; Kobuvirus; Nutrition; Oral administration; Polymerase chain reaction; Real time; Research Article; Reverse transcription; Sewage; Surface water; Water pollution; 식품과학; Real-time RT-PCR; Aichivirus; Conventional RT-PCR; Human gastroenteritis; Foodborne viruses
• ISSN: 1226-7708; 2092-6456; 2092-6456
• DOI: 10.1007/s10068-024-01537-9

Aichivirus-A (AiV-A), a member of the Kobuvirus genus of the family Picornaviridae, was first reported in stool samples of patients with non-bacterial gastroenteritis in Aichi Prefecture, Japan, in 1989. AiV has been reported from in various aquatic environments, such as surface water and sewage, can be transmitted via the fecal–oral route through contaminated water. As AiV is known to acute gastroenteritis worldwide, developing methods for AiV detection from contaminated environments and food is required. In the present study, we established an effective polymerase chain reaction (PCR) method to detect AiV. Various real-time reverse transcription (RT)-PCR and conventional PCR methods for AiV detection were compared, and the limit of detection was confirmed by comparing the sensitivity at varied primer concentrations and PCR conditions. The final detection limits were 10 2 copy/μL in conventional PCR, and 10 1 copy/μL in the real-time RT-PCR. The optimized method used in this study might aid in detecting AiV contamination.