New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes

• Published in: International journal of molecular sciences Vol. 22; no. 4; p. 2082
• Main Authors: Kulus, Magdalena, Kranc, Wiesława, Wojtanowicz-Markiewicz, Katarzyna, Celichowski, Piotr, Światły-Błaszkiewicz, Agata, Matuszewska, Eliza, Sujka-Kordowska, Patrycja, Konwerska, Aneta, Zdun, Maciej, Bryl, Rut, Wieczorkiewicz, Maria, Kulus, Jakub, Stelmach, Bogusława, Stefańska, Katarzyna, Budna-Tukan, Joanna, Petitte, James N, Mozdziak, Paul, Ratajczak, Kornel, Matysiak, Jan, Jaśkowski, Jędrzej M, Nowicki, Michał, Kempisty, Bartosz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 19.02.2021; MDPI AG
• Subjects: Animals; Cell Differentiation - genetics; Cell Shape - genetics; Cells, Cultured; Down-Regulation - genetics; epithelial cells; Epithelial Cells - metabolism; Female; Gene Expression Regulation; Gene Ontology; Gene Regulatory Networks; Genetic Markers; long-term in vitro culture; microarray assays; Oocytes - metabolism; oviduct; Oviducts - cytology; pig; Signal Transduction - genetics; Swine; Transcriptome; transcriptomics; Up-Regulation - genetics; microarray assays; oviduct; proteomics; transcriptomics; epithelial cells; long-term in vitro culture; pig
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms22042082

Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in 'cell development', 'cell growth', 'cell differentiation' and 'cell maturation' processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of and downregulation of . Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.