Liquid chromatographic determination of sugar alcohols in beverages and foods after nitrobenzoylation

Use of p-nitrobenzoyl chloride (PNBC) to form an ultraviolet-absorbing derivative was attempted to determine the sugar alcohols meso-erythritol, xylitol, D-sorbitol, and D-mannitol by liquid chromatography (LC). LC determination of derivatives was performed on an ODS column with acetonitrile-water (...

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Bibliographic Details
Published inJournal of AOAC International Vol. 82; no. 1; pp. 134 - 140
Main Authors Nojiri, S, Saito, K, Taguchi, N, Oishi, M, Maki, T
Format Journal Article
LanguageEnglish
Published England 1999
Subjects
benzoates
beverages
Beverages - analysis
Calibration
Chromatography, Liquid
erythritol
Food Analysis
food composition
foods
Hot Temperature
Linear Models
liquid chromatography
mannitol
Nitrobenzenes - chemistry
quantitative analysis
Sensitivity and Specificity
sorbitol
Sugar Alcohols - analysis
xylitol
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Summary:Use of p-nitrobenzoyl chloride (PNBC) to form an ultraviolet-absorbing derivative was attempted to determine the sugar alcohols meso-erythritol, xylitol, D-sorbitol, and D-mannitol by liquid chromatography (LC). LC determination of derivatives was performed on an ODS column with acetonitrile-water (65 + 35) as mobile phase. Calibration curves were linear in the concentration range 0.01-100 microgram/mL. Method sensitivity is 10 to 1000 times higher than that of LC with refractive index detection and gas chromatography with flame ionization detection. Recoveries of sugars added to various foods at 0.1 and 1% ranged from 91 to 102% for mesoerythritol, 75 to 115% for xylitol, 81 to 105% for D-sorbitol, and 81 to 108% for D-mannitol.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/82.1.134