A comparative study of ethylene oxide gas, hydrogen peroxide gas plasma, and low-temperature steam formaldehyde sterilization

• Published in: Infection control and hospital epidemiology Vol. 26; no. 5; p. 486
• Main Authors: Kanemitsu, Keiji, Imasaka, Takayuki, Ishikawa, Shiho, Kunishima, Hiroyuki, Harigae, Hideo, Ueno, Kumi, Takemura, Hiromu, Hirayama, Yoshihiro, Kaku, Mitsuo
• Format: Journal Article
• Language: English
• Published: United States 01.05.2005
• Subjects: Equipment and Supplies - microbiology; Ethylene Oxide; Formaldehyde; Geobacillus stearothermophilus - physiology; Hydrogen Peroxide; Japan; Spores, Bacterial; Sterilization - methods; Temperature; Japan
• ISSN: 0899-823X
• DOI: 10.1086/502572

To compare the efficacies of ethylene oxide gas (EOG), hydrogen peroxide gas plasma (PLASMA), and low-temperature steam formaldehyde (LTSF) sterilization methods. The efficacies of EOG, PLASMA, and LTSF sterilization were tested using metal and plastic plates, common medical instruments, and three process challenge devices with narrow lumens. All items were contaminated with Bacillus stearothermophilus spores or used a standard biological indicator. EOG and LTSF demonstrated effective killing of B. stearothermophilus spores, with or without serum, on plates, on instruments, and in process challenge devices. PLASMA failed to adequately sterilize materials on multiple trials in several experiments, including two of three plates, two of three instruments, and all process challenge devices. Our results suggest that PLASMA sterilization may be unsuccessful under certain conditions, particularly when used for items with complex shapes and narrow lumens. Alternatively, LTSF sterilization demonstrates excellent efficacy and is comparable to EOG sterilization. LTSF could potentially act as a substitute if EOG becomes unavailable due to environmental concerns.