Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices

• Published in: Chemical communications (Cambridge, England) Vol. 54; no. 99; pp. 13977 - 13980
• Main Authors: Wang, Liang, Chen, Xiaohan, Zhou, Shuo, Roozbahani, Golbarg M., Zhang, Youwen, Wang, Deqiang, Guan, Xiyun
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 11.12.2018
• Subjects: Biological properties; Biomarkers - blood; Biosensing Techniques - methods; blood; chemical compounds; Chemical reactions; Clinical Chemistry Tests - methods; Diagnostic software; Diagnostic systems; Displacement; DNA Probes - chemistry; DNA, Single-Stranded - chemistry; Humans; MicroRNAs - blood; MicroRNAs - chemistry; Nanopores; Nucleic Acid Hybridization; Nucleic acids; Organic chemistry; Point-of-Care Systems; Porosity; Proof of Concept Study; Stochastic Processes

To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high sensitivity for detecting nucleic acids, our develop...