Displacement chemistry-based nanopore analysis of nucleic acids in complicated matrices

To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high sensitivity for detecting nucleic acids, our develop...

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Bibliographic Details
Published inChemical communications (Cambridge, England) Vol. 54; no. 99; pp. 13977 - 13980
Main Authors Wang, Liang, Chen, Xiaohan, Zhou, Shuo, Roozbahani, Golbarg M., Zhang, Youwen, Wang, Deqiang, Guan, Xiyun
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 11.12.2018
Subjects
Biological properties
Biomarkers - blood
Biosensing Techniques - methods
blood
chemical compounds
Chemical reactions
Clinical Chemistry Tests - methods
Diagnostic software
Diagnostic systems
Displacement
DNA Probes - chemistry
DNA, Single-Stranded - chemistry
Humans
MicroRNAs - blood
MicroRNAs - chemistry
Nanopores
Nucleic Acid Hybridization
Nucleic acids
Organic chemistry
Point-of-Care Systems
Porosity
Proof of Concept Study
Stochastic Processes
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Summary:To overcome the effect of other components of complicated biological samples on nanopore stochastic sensing, displacement chemical reaction was utilized to selectively extract the target nucleic acid from whole blood. Given its simplicity and high sensitivity for detecting nucleic acids, our developed displacement chemistry-based nanopore sensing strategy offers the potential for fieldable/point-of-care diagnostic applications.
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ISSN:1359-7345
1364-548X
1364-548X
DOI:10.1039/C8CC07944G