Overexpression of a Malus baccata NAC Transcription Factor Gene MbNAC25 Increases Cold and Salinity Tolerance in Arabidopsis
NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are availabl...
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Published in | International journal of molecular sciences Vol. 21; no. 4; p. 1198 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI
11.02.2020
MDPI AG |
Subjects | |
Online Access | Get full text |
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Summary: | NAC (no apical meristem (NAM), Arabidopsis thaliana transcription activation factor (ATAF1/2) and cup shaped cotyledon (CUC2)) transcription factors play crucial roles in plant development and stress responses. Nevertheless, to date, only a few reports regarding stress-related NAC genes are available in
Borkh. In this study, the transcription factor
in
.
was isolated as a member of the plant-specific NAC family that regulates stress responses. Expression of
was induced by abiotic stresses such as drought, cold, high salinity and heat. The ORF of
is 1122 bp, encodes 373 amino acids and subcellular localization showed that MbNAC25 protein was localized in the nucleus. In addition,
was highly expressed in new leaves and stems using real-time PCR. To analyze the function of
in plants, we generated transgenic
plants that overexpressed
Under low-temperature stress (4 °C) and high-salt stress (200 mM NaCl), plants overexpressing
enhanced tolerance against cold and drought salinity conferring a higher survival rate than that of wild-type (WT). Correspondingly, the chlorophyll content, proline content, the activities of antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were significantly increased, while malondialdehyde (MDA) content was lower. These results indicated that the overexpression of
in
plants improved the tolerance to cold and salinity stress via enhanced scavenging capability of reactive oxygen species (ROS). |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1422-0067 1422-0067 |
DOI: | 10.3390/ijms21041198 |