Sleep deprivation prevents stimulation-induced increases of levels of P-CREB and BDNF: Protection by caffeine

• Published in: Molecular and cellular neuroscience Vol. 46; no. 4; pp. 742 - 751
• Main Authors: Alhaider, Ibrahim A., Aleisa, Abdulaziz M., Tran, Trinh T., Alkadhi, Karim A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2011
• Subjects: Animals; Brain - drug effects; Brain - metabolism; Brain-Derived Neurotrophic Factor - metabolism; CA1 area; Caffeine; Caffeine - pharmacology; Cyclic AMP Response Element-Binding Protein - genetics; Cyclic AMP Response Element-Binding Protein - metabolism; Hippocampus - drug effects; Hippocampus - metabolism; Humans; Lat-long-term potentiation; Long-Term Potentiation - drug effects; Long-Term Potentiation - physiology; Male; Memory - drug effects; Memory Disorders - etiology; P-CREB; Phosphorylation; Rats; Rats, Wistar; Sleep deprivation; Sleep Deprivation - complications; Sleep Deprivation - physiopathology; Spatial memory; CA1 area; Lat-long-term potentiation; P-CREB; Sleep deprivation; Caffeine; Spatial memory
• ISSN: 1044-7431; 1095-9327
• DOI: 10.1016/j.mcn.2011.02.006

It is well known that caffeine and sleep deprivation have opposing effects on learning and memory; therefore, this study was undertaken to determine the effects of chronic (4 wks) caffeine treatment (0.3 g/l in drinking water) on long-term memory deficit associated with 24 h sleep deprivation. Animals were sleep deprived using the modified multiple platform method. The results showed that chronic caffeine treatment prevented the impairment of long-term memory as measured by performance in the radial arm water maze task and normalized L-LTP in area CA1 of the hippocampi of sleep-deprived anesthetized rats. Sleep deprivation prevents the high frequency stimulation-induced increases in the levels of phosphorylated-cAMP response element binding protein (P-CREB) and brain-derived neurotrophic factor (BDNF) seen during the expression of late phase long-term potentiation (L-LTP). However, chronic caffeine treatment prevented the effect of sleep-deprivation on the stimulated levels of P-CREB and BDNF. The results suggest that chronic caffeine treatment may protect the sleep-deprived brain probably by preserving the levels of P-CREB and BDNF.