Evidence of Methylobacterium spp. and Hyphomicrobium sp. in azaspiracid toxin contaminated mussel tissues and assessment of the effect of azaspiracid on their growth

• Published in: Toxicon (Oxford) Vol. 58; no. 8; pp. 619 - 622
• Main Authors: Nzoughet, Judith K., Grant, Irene R., Prodöhl, Paulo A., Hamilton, John T.G., Botana, Luis M., Elliott, Christopher T.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.12.2011; Elsevier
• Subjects: Agrobacterium; Animal poisons toxicology. Antivenoms; Animals; Assessments; azaspiracid; Azaspiracids; Biological and medical sciences; Chromatography, High Pressure Liquid; coculture; Culture; Density; DNA sequencing; Foodborne Diseases - microbiology; Hyphomicrobium; Hyphomicrobium - drug effects; Hyphomicrobium - growth & development; Hyphomicrobium - isolation & purification; Hyphomicrobium sp; Marine; Marine Toxins - analysis; Marine Toxins - pharmacology; Medical sciences; Methylobacterium; Methylobacterium - drug effects; Methylobacterium - growth & development; Methylobacterium - isolation & purification; Methylobacterium spp; Microbial Viability - drug effects; Mussels; Mytilus edulis - chemistry; Mytilus edulis - microbiology; PCR; Phylogenetics; Proteomics; Spiro Compounds - analysis; Spiro Compounds - pharmacology; Tandem Mass Spectrometry; tissues; Toxic; toxicity; Toxicology; Toxins; Phylogenetics; Azaspiracids; Methylobacterium spp; Hyphomicrobium sp; PCR; DNA sequencing; Mussel; Nucleotide sequence; Phylogenetic tree; Hyphomicrobiaceae; Methylobacterium; Polymerase chain reaction; Tissue; Toxin; Bivalvia; DNA; Bacteria; Hyphomicrobiales; Invertebrata; Mollusca; Sequencing; Hyphomicrobium
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2011.09.012

A flagellar protein belonging to the genus Methylobacterium or Agrobacterium was previously observed by proteomics in azaspiracids (AZA) toxic mussels. Here, we report the isolation of two different Methylobacterium spp. (NTx1 and Tx1) from non-toxic and AZA toxic mussels, respectively, which when co-cultured with AZA exhibited significantly different growth responses - isolate Tx1 growth rate was enhanced, whereas growth of isolate NTx1 was adversely affected, compared to non-AZA supplemented control cultures. A Hyphomicrobium sp. (Tx2) also isolated from the toxic mussels achieved greater cell density in AZAs supplemented cultures.