A new conformation-specific infrared band of A-DNA in films

A band at 1185 cm−1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm−1 band has...

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Bibliographic Details
Published inNucleic acids research Vol. 8; no. 11; pp. 2527 - 2536
Main Authors Pohle, Walter, Fritzsche, Hartmu
Format Journal Article
LanguageEnglish
Published England Oxford University Press 11.06.1980
Subjects
Animals
Caffeine
Cattle
DNA
Nucleic Acid Conformation
Spectrophotometry, Infrared
Thymus Gland
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Summary:A band at 1185 cm−1 occurs in the infrared spectra of nucleic acids in their A-type conformation which has been reproduced in the literature, too. The absorbance of this band is proportional to the fraction of the A form of DNA samples containing a mixture of different forms. The 1185 cm−1 band has been assigned tentatively to a vibration of the sugar-phosphate backbone with a fairly high contribution from the sugar moiety. Drastic dehydration of the DBA films is accompanied by a continuous intensity decrease of the 1185 cm−1 band indicating a collapse of the A form.
Bibliography:istex:B6C1B36CDB32E22DE006D5E63D11193EE299192D
ArticleID:8.11.2527
ark:/67375/HXZ-SDD0V271-T
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/8.11.2527