Optical detection of nanoparticle-enhanced human papillomavirus genotyping microarrays

• Published in: Biomedical optics express Vol. 4; no. 2; pp. 187 - 192
• Main Authors: Li, Xue Zhe, Kim, Sookyung, Cho, Wonhyung, Lee, Seung-Yop
• Format: Journal Article
• Language: English
• Published: United States Optical Society of America 01.02.2013
• Subjects: Biosensors and Molecular Diagnostics; (170.3890) Medical optics instrumentation; (170.3880) Medical and biological imaging; (170.4580) Optical diagnostics for medicine
• ISSN: 2156-7085; 2156-7085
• DOI: 10.1364/boe.4.000187

In this study, we propose a new detection method of nanoparticle-enhanced human papillomavirus genotyping microarrays using a DVD optical pick-up with a photodiode. The HPV genotyping DNA chip was labeled using Au/Ag core-shell nanoparticles, prepared on a treatment glass substrate. Then, the bio information of the HPV genotyping target DNA was detected by measuring the difference of the optical signals between the DNA spots and the background parts for cervical cancer diagnosis. Moreover the approximate linear relationship between the concentration of the HPV genotyping target DNA and the optical signal depending on the density of Au/Ag core-shell nanoparticles was obtained by performing a spot finding algorithm. It is shown that the nanoparticle-labeled HPV genotyping target DNA can be measured and quantified by collecting the low-cost photodiode signal on the treatment glass chip, replacing high-cost fluorescence microarray scanners using a photomultiplier tube.