Renal aminoacylase, a zinc enzyme

Renal aminoacylase is inactivated by dialysis against metal complexing agents such as o-phenanthroline. Activity can be restored by addition of zinc ions. A zinc dissociation constant of about 10(-10)M at pH 7.8 is obtained by titration of the enzyme with a metal ion buffer. The reactivity of the SH...

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Bibliographic Details
Published inZeitschrift für Naturforschung. Section C, Biosciences Vol. 32; no. 5-6; pp. 342 - 344
Main Authors Kördel, W, Schneider, F
Format Journal Article
LanguageEnglish
Published Germany 01.06.1977
Subjects
Amidohydrolases - analysis
Amidohydrolases - antagonists & inhibitors
Amidohydrolases - metabolism
Animals
Binding Sites
Cysteine - metabolism
Kidney - enzymology
Norleucine - pharmacology
Swine
Zinc - analysis
Zinc - metabolism
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Summary:Renal aminoacylase is inactivated by dialysis against metal complexing agents such as o-phenanthroline. Activity can be restored by addition of zinc ions. A zinc dissociation constant of about 10(-10)M at pH 7.8 is obtained by titration of the enzyme with a metal ion buffer. The reactivity of the SH groups of the enzyme is considerably affected by zinc ions. The enzyme contains two essential zinc ions per molecule.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0341-0382
DOI:10.1515/znc-1977-5-605