Suppression of the Antioxidant System and PI3K/Akt/mTOR Signaling Pathway in Cisplatin-Resistant Cancer Cells by Quercetin

• Published in: Bulletin of experimental biology and medicine Vol. 173; no. 6; pp. 760 - 764
• Main Authors: Hasan, A. A. Sh, Kalinina, E. V., Tatarskiy, V. V., Volodina, Yu. L., Petrova, А. S., Novichkova, M. D., Zhdanov, D. D., Shtil, A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2022; Springer; Springer Nature B.V
• Subjects: 1-Phosphatidylinositol 3-kinase; Adenocarcinoma; AKT protein; Antioxidants; Antitumor agents; Apoptosis; Biomedical and Life Sciences; Biomedicine; Cancer; Cancer cells; Cell Biology; Cell cycle; Cell viability; Chemotherapy; Cisplatin; Drug resistance; Enzymes; Flavonoids; Free radicals; Gene expression; Genes; Internal Medicine; Kinases; Laboratory Medicine; Ovaries; Pathology; Quercetin; Signal transduction; Software; TOR protein; quercetin; drug resistance of tumor cells; gene expression; PI3K-AkT-mTOR signaling pathway
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-022-05626-9

We studied the effect of quercetin on ovarian adenocarcinoma SKOV-3 cell line and isogenic subline SKOV-3/CDDP resistant to the anticancer drug cisplatin. It was found that in resistant cells, quercetin in a concentration of 100 μM that causes a decrease in the cell viability suppressed the expression of genes encoding the key antioxidant enzymes ( SOD2 , CAT , GPX1 , and HO-1 ), transcription factor Nrf2, and kinases of the PI3K/Akt/mTOR signaling pathway. In parental cells, quercetin, on the contrary, increased the expression of these genes. The results confirm the redox-dependent regulation induced by quercetin and its opposite nature in cisplatin-sensitive and cisplatin-resistant cancer cells.